![IGEM:Hong Kong HKUST/Investigations/The Effect of the Vector to Insert DNA Ratio on the Efficiency of Ligation - OpenWetWare IGEM:Hong Kong HKUST/Investigations/The Effect of the Vector to Insert DNA Ratio on the Efficiency of Ligation - OpenWetWare](https://s3-us-west-2.amazonaws.com/oww-files-thumb/4/41/Alice_Wang_Table1.png/700px-Alice_Wang_Table1.png)
IGEM:Hong Kong HKUST/Investigations/The Effect of the Vector to Insert DNA Ratio on the Efficiency of Ligation - OpenWetWare
![SOLVED: 1. Calculate the volume of plasmid and insert needed for a ligation reaction using the specifications for NEBL T4 DNA ligase (0.020 pmol vector, 0.060 pmol insert). This reaction is ligating SOLVED: 1. Calculate the volume of plasmid and insert needed for a ligation reaction using the specifications for NEBL T4 DNA ligase (0.020 pmol vector, 0.060 pmol insert). This reaction is ligating](https://cdn.numerade.com/ask_previews/8571bce7-c922-4656-ac07-a4fd2d5f4bd7_large.jpg)
SOLVED: 1. Calculate the volume of plasmid and insert needed for a ligation reaction using the specifications for NEBL T4 DNA ligase (0.020 pmol vector, 0.060 pmol insert). This reaction is ligating
![SOLVED: order follox Inser Vector molar ntic ligatinn the insert and 02 pmo aCrcr ddec microcertrifuga FUDE with TA DNA ligase , appropnate buffcr ciHzo Why Impanant More molecules nsen ector Tu SOLVED: order follox Inser Vector molar ntic ligatinn the insert and 02 pmo aCrcr ddec microcertrifuga FUDE with TA DNA ligase , appropnate buffcr ciHzo Why Impanant More molecules nsen ector Tu](https://cdn.numerade.com/ask_images/b9265470273a4da18f28b4843c0f8b05.jpg)
SOLVED: order follox Inser Vector molar ntic ligatinn the insert and 02 pmo aCrcr ddec microcertrifuga FUDE with TA DNA ligase , appropnate buffcr ciHzo Why Impanant More molecules nsen ector Tu
![Ligation Protocol Document - Ligation Protocol Kit name: T4 DNA ligase, From: Thermofisher You are - Studocu Ligation Protocol Document - Ligation Protocol Kit name: T4 DNA ligase, From: Thermofisher You are - Studocu](https://d20ohkaloyme4g.cloudfront.net/img/document_thumbnails/144f247f5e12a2274694468dd929fd5a/thumb_1200_1553.png)
Ligation Protocol Document - Ligation Protocol Kit name: T4 DNA ligase, From: Thermofisher You are - Studocu
![How to calculate Molar Ratio of Insert:Vector for Ligation in Excel for cloning DNA fragments - YouTube How to calculate Molar Ratio of Insert:Vector for Ligation in Excel for cloning DNA fragments - YouTube](https://i.ytimg.com/vi/MIhjaIogHNg/mqdefault.jpg)